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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was identified to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a important part in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but thorough nucleotide sequence info will not be but available.
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Blocking peptides are peptides that bind particularly for the goal antibody and block antibody binding. These peptide commonly has the epitope recognized through the antibody.
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This information outlines more feasible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This tutorial outlines further more feasible optimization of cellular and immunoassay parameters to make sure the best possible final results are...
Antibodies certain to the blocking peptide not bind for the epitope around the goal protein. By evaluating the staining in the blocked antibody compared to the antibody by itself, one can see which staining is distinct.
Focus on Data Cyclin E belongs for the very conserved cyclin loved ones, whose members are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which add for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities as being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S stage boundary and it is degraded as cells progress through S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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